HighEnd™ Repair Kit

highQu Cat.No.HER0101Size: 40 reactions


• Preparation of PCR products, shared/nebulized, restriction-digested DNA, cDNA for blunt-end ligation
• Conversion of 5′- and/or 3′-protruding ends to 5′-phosphorylated blunt-ended ones


• Fast and simple blunting and phosphorylation of DNA at once
• Universal - preparing any kind of DNA for blunt-end ligation
• Premium reagents - reproducible results


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HighEnd™ Repair Kit is a premium tool designed for rapid and highly efficient DNA end-repair before the ligation reactions. PCR products, shared or nebulized DNA, restriction-digested DNA and cDNA can be blunted/phosphorylated in a couple of minutes and are ready for an efficient blunt-end ligation and cloning. The Kit includes HighEnd™ Repair Blend – an optimized mix of T4 DNA Polymerase and T4 Polynucleotide Kinase. The 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase form the blunt-ended DNA. T4 Polynucleotide Kinase phosphorylates 5′ DNA ends. The resulting DNA is a high quality blunt-ended substrate for T4 DNA Ligase. Up to 1-5 microgram of the linear DNA can be blunted and phosphorylated in one 20 min reaction. After the thermal inactivation the reaction mixture can be used for blunt-end ligations. HighEnd™ Repair Kit is an ideal choice for preparing for ligations the PCR products obtained with high fidelity polymerases like ALLin™ HiFi DNA Polymerase (HLE0201).
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With headquarters in Germany and a rapidly growing distributor network reaching more than 15 countries, a young but experienced company highQu supports life science worldwide by providing premium quality, outperforming products for PCR, real-time PCR, RT-PCR, electrophoresis and other molecular biology applications. highQu is determined to supply professionally simple products – minimum optimization, fast and easy procedures, short protocols, outstanding performance in both professional and student hands.