HighEnd™ Repair Kit

highQu via Clent Life Science Cat.No.HER0101Size: 40 reactions


• Preparation of PCR products, shared/nebulized, restriction-digested DNA, cDNA for blunt-end ligation
• Conversion of 5′- and/or 3′-protruding ends to 5′-phosphorylated blunt-ended ones


• Fast and simple blunting and phosphorylation of DNA at once
• Universal - preparing any kind of DNA for blunt-end ligation
• Premium reagents - reproducible results


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HighEnd™ Repair Kit is a premium tool designed for rapid and highly efficient DNA end-repair before the ligation reactions. PCR products, shared or nebulized DNA, restriction-digested DNA and cDNA can be blunted/phosphorylated in a couple of minutes and are ready for an efficient blunt-end ligation and cloning. The Kit includes HighEnd™ Repair Blend – an optimized mix of T4 DNA Polymerase and T4 Polynucleotide Kinase. The 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase form the blunt-ended DNA. T4 Polynucleotide Kinase phosphorylates 5′ DNA ends. The resulting DNA is a high quality blunt-ended substrate for T4 DNA Ligase. Up to 1-5 microgram of the linear DNA can be blunted and phosphorylated in one 20 min reaction. After the thermal inactivation the reaction mixture can be used for blunt-end ligations. HighEnd™ Repair Kit is an ideal choice for preparing for ligations the PCR products obtained with high fidelity polymerases like ALLin™ HiFi DNA Polymerase (HLE0201).
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With headquarters in Germany and a rapidly growing distributor network reaching more than 15 countries, highQu supports life science worldwide by providing premium quality, outperforming products for PCR, real-time PCR, RT-PCR, electrophoresis and other molecular biology applications. highQu is determined to supply professionally simple products – minimum optimization, fast and easy procedures, short protocols, outstanding performance in both professional and student hands.

Clent Life Science is the authorized distributor of highQu premium tools for molecular biology in the United Kingdom.

Clent Life Science successfully serves researchers in life science throughout the UK for more than 5 years. The company works with a selected list of forward thinking suppliers to bring best products to the life science industry. The reagents and instruments of Clent Life Science are well documented for DNA extraction and PCR, HRM and bench top performance in molecular and cellular biology.