SampleIN™ Direct PCR Kit
highQu Cat.No.DPK010SSize: 1 sample size
Direct PCR without template purification
from mouse tail, ear, mammalian tissue (FFPE),
hair follicle, buccal swab, plants, blood (EDTA, FTA).
• PCR in 50 min without template purification
• Single-tube 15 min DNA extraction combined with fast hot-start PCR
• Red dye for direct loading
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SampleIN™ Direct PCR Kit is a premium tool for a fast direct PCR eliminating the need of tedious template purification. The kit is excellent for direct PCR from mouse tail or ear, mammalian tissues, hair follicle, buccal swabs and blood. Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading. In a 2% agarose TAE gel the red dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The ALLin™ HS Red Taq Mastermix includes a hot start Taq DNA Polymerase what ensures high yield, specific, low background amplification. Mix components allow for a fast PCR cycling and increase success when working with complex templates or multiplexing. Generated A-tailed PCR products are suitable for ligating into TA cloning vectors, sequencing and other applications.
With headquarters in Germany and a rapidly growing distributor network reaching more than 15 countries, a young but experienced company highQu supports life science worldwide by providing premium quality, outperforming products for PCR, real-time PCR, RT-PCR, electrophoresis and other molecular biology applications. highQu is determined to supply professionally simple products – minimum optimization, fast and easy procedures, short protocols, outstanding performance in both professional and student hands.