Affinity chromatography is one of the main methods used for protein purification. Besides chromatography, the affinity tags are used for antibody-based detection of tagged proteins, for protein immobilization on ELISA plates or other surfaces, and for pulldown of proteins from complex solutions.

The decision for the right affinity tag has to be taken long before protein expression; at the stage of constructing the recombinant gene with appropriate tag-coding sequence.

Choosing the right affinity tag guaranties a big portion of success in your protein purification workflow. How not to get lost in the variety of commercial affinity tags and different chromatography resins?

Select it thoroughly by following the advice of experts from Cube Biotech. With the right chromatography resin your protein purification will be successfully finished after three general steps of binding, washing and elution.

According to Cube Biotech, in addition to the nature of affinity tag, many other parameters are important to consider when choosing the right resin. The specificity of interaction, elution conditions and the expected protein yield are among such parameters.

Every tag has its best application area. For example, His tag is good for soluble proteins (both native and denaturing purification conditions), whereas the Rho1D4 tag allows to purify membrane proteins with high specificity under the mild elution conditions. GST tag enhances protein solubility, promotes protein folding and is especially useful for pulldown experiments.

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PureCube Glutathione Agarose samples @ flash4science are offered for two weeks in US, UK and Germany.

Image  by Max Williams  / CC BY 2.0

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