Viromer® transfection: the impossible made possible
The article has been prepared based on materials provided by Lipocalyx the Viromer company.
Successful transfection is the key enabling gene function and regulation analysis, helping to investigate cellular pathways and signal transduction mechanisms in cells, tissues and eukaryotic organisms. Even though there are various viral, physical and chemical transfection methods developed, the successful transfection of certain difficult-to-transfect cells remains a challenging task. Luckily, since a couple of years, the novel Viromer® transfection method featuring an active liposome escape mechanism became the outstanding solution for many laboratories working with challenging cell lines.
Picture1: primary human pancreatic tumor cells, patient derived
Lipocalyx proprietary synthetic transfection reagents Viromer® combine the best features of chemical transfection and mimic the natural viral transfection mechanism to enable an outstanding transfer of RNAs and DNA into various hard-to-transfect cells. Viromer® are unique, polymer-based transfection reagents free of lipids and free of animal or viral compounds. They form stable particles complexed with nucleic acids (NA), which are taken up by endocytosis. When exposed to low pH in the endosome, Viromer®-DNA/RNA complexes loose their charge, become hydrophobic and cross the endosomal membrane. Viromer® reagents thus feature an active endosome escape mechanism. The neutral charge of the particles is a unique feature of the Viromer® technology and results in full compatibility with serum, antibiotics and a very gentle interaction with the cells, in particular when grown in suspension. Once applied, Viromer®:NA complexes can be left on the cells and no further washes or media changes are required.
Due to their easy cell entry mechanism and the extremely good tolerability, Viromer® allow for a highly efficient transfection of eucaryotic cells and outperform other transfection reagents. Differences become most recognizable in difficult-to-transfect cells, such as primary cells, stem cells, macrophages, myocytes, hepatocytes and certain neuronal cells.
Picture 2: C6 glioma
Viromer® RED in vitro pDNA/mRNATransfection Reagent is a superior tool for an efficient delivery of plasmid DNA/shRNA/mRNA without the use of enhancers or tedious optimizations into hard-to-transfect cells such as phagocytes, primary cardiomyocytes and related muscle cells, primary monocytes and macrophages, primary fibroblasts or keratinocytes, suspension or stem cells.
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Summarized benefits of Viromer® transfection reagents:
- High transfection efficiency achievable even in hard-to-transfect cells
- High cell viability even in monocytes and other sensitive cells
- Multipurpose reagent for pDNA and mRNA, forward and reverse transfection
- Easy-to-use - no changes of media, no cell washes, no or little optimization
- Versatile - compatible with the use of serum or antibiotics
- Convenient - no additives required
- Economical - only 1 µl for transfection of 0,5 µg of pDNA or mRNA in a 24 format well
- Time-saving – fast procedure and straightforward workflow
Picture 3: mRNA transfection in primary human monocytes
Picture 4: SH-SY5Y neuroblastoma